Human Interleukin 33 (IL-33) ELISA kit

Human Interleukin 33 (IL-33) FOR RESEARCH USE ONLY Assay range：100pg/ml-2000pg/ml 96 determinations Purpose This kit allows for the determination of IL-33 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human IL-33 level in the sample, use Purified Human IL-33 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-33 to wells, Combined IL-33 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.