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Human KRAS FOR RESEARCH USE ONLY Assay range:60 ng/L -2000 ng/L 96 determinations Purpose This kit allows for the determination of KRAS concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay Human KRAS level in the sample, use Purified Human KRAS antibody to coat microtiter plate wells, make solid-phase antibody, then add KRAS to wells, Combined KRAS antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human KRAS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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