Human IL-28 ELISA kit

Human IL-28 uman IL-28FOR RESEARCH USE ONLY Assay range：6pg/ml-280pg/ml 96 determinations Purpose This kit allows for the determination of IL-28 concentrations in Human serum, cell culture supernates and other biological fluids Principle of the assay The kit assay HumanIL-28 level in the sample，use Purified Human IL-28 antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-28 to wells, Combined IL-28 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human IL-28 in the samples is then determined by comparing the O.D. of the samples to the standard curve.