SFC 新技术SFC应用

Even though Supercritical Fluid Chromatography (SFC) was first demonstrated by Klesper et al. in 1962, 6 – 7 years earlier than High-Performance Liquid Chromatography (HPLC), its history is unfortunate and it has never been a main stream of chromatography. Why?Partly because an automated liquid chromatograph (LC), namely, an amino acid analyzer by Spackman, Stein and Moore, had already been commercially available in the late 1950’s and there were actual needs for LC, while therewere no such needs for SFC except for some exotic samples such as metalloporphyrins and metallic chelates at that time. Rapid development of HPLC occurred in the 1970’s overshadowed the development of SFC taken place at the same time. However, there are remarkable research works done in the 1960’s, the 1970’s and the 1980’s that realized the today’s modern SFC, and this poster will review these works.