小鼠内毒素（ET）Elisa试剂盒

小鼠内毒素（ET）Elisa试剂盒 Mouse endotoxin FOR RESEARCH USE ONLY Assay range：0.02Eu/L – 0.8Eu/L 96 determinations Purpose This kit allows for the determination of Endotoxin concentrations in Mouse serum. Principle of the assay The kit assay Mouse Endotoxin level in the sample, use Purified Mouse Endotoxin antibody to coat microtiter plate wells, make solid-phase antibody, then add Endotoxin to wells, Combined Endotoxin antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse Endotoxin in the samples is then determined by comparing the O.D. of the samples to the standard curve.