液相分析固醇类固定相的选择---色谱柱选择

Steroids are an important class of pharmaceuticals. Of the top 200 prescribed drugs in 1998, 29 are classified as steroids.1 Their uses range from contraception and hormone therapy to treatment of bronchial asthma. Steroids of differing sub¬classes frequently are used in combinations to generate a desired therapeutic effect. High performance liquid chromatography (HPLC) analysis of steroids can be performed with many types of stationary phases. Restek’s Ultra C18, Ultra C4, Ultra Cyano, Allure™ C18, and Allure™ Basix columns can provide the selectivity and retention characteristics needed to develop rapid and robust analytical methods for a wide range of steroids. Therefore, the choice of column phase essentially is dictated by the sample matrix and sensitivity concerns.