同时在奥特IBD C18上分析核苷，生化（核苷），嘌呤，咖啡碱，嘧啶

侯先生 13691497252 Mixtures of nucleotides, nucleosides, and their respective purine or pyrimidine bases are difficult to analyze by reversed phase/high performance liquid chromatography (RP/HPLC). These compounds cover a wide range of polarities and functionalities, from the acidic nucleotides to the basic purines and pyrimidines, making it very difficult to retain and resolve all of them with conventional alkyl stationary phases. Traditional HPLC analysis of these compounds often uses a combination of reversed phase-ion pairing (RP-IP) and/or ion exchange (IEX) mode. Nucleotides often are analyzed by anion exchange, while nucleosides sometimes are analyzed by cation exchange. These methods are not compatible with all the solutes in the mixtures and they lack ruggedness. This Applications Note demonstrates that all three classes of compounds (nucleotides, nucleosides, and bases) can be analyzed by RP/HPLC using one column and the same, simple isocratic mobile phase. This provides greater convenience, reproducibility, and ruggedness in developing methods for these mixtures. By using a unique, intrinsically base-deactivated stationary phase (i.e., the Ultra IBD column), simple RP/HPLC conditions were identified that can resolve any common purine or pyrimidine base from its related ribonucleoside and mono-, di-, and triphosphate nucleotides. Nucleotides and nucleosides are derived from the nitrog¬enous bases shown in Figure 1. These nitrogenous bases are either purines (adenine and guanine) or pyrimidines (cytosine, uracil, and thymine). A nucleoside consists of a purine or pyrimidine base linked to a five-carbon sugar (pentose). A nucleotide is composed of a nucleoside plus one or more phosphate groups. Figure 2 shows the structures of a nucleoside and three nucleotides derived from adenine.