用HPLC分析强心药物异羟洋地黄苷方法

侯先生 13691497252 In the United States, cardiovascular disease is the leading cause of death. In an effort to reduce mortality from heart disease, several classes of medications are used to decrease high blood pressure, control arrhythmias (abnormal heart rhythms), and treat congestive heart failure. Many of these cardiac medications include beta antagonists, ACE inhibi¬tors, diuretics, or calcium channel blockers. High performance liquid chromatography (HPLC) is the preferred technique to analyze many of the compounds used in these medications. To maximize the effectiveness of the separation, a chromatographer should choose the column and conditions that best allow amplification of structural differences between matrix components, related compounds, and analytes. Proper HPLC column selection is dictated by the analyte and the sample matrix. In fact, selecting the appropriate analytical column is critical when analyzing cardiac medications because many of them contain basic compounds, which tend to tail badly on poorly deactivated HPLC phases. Restek’s fully end-capped Allure™ Basix, Ultra IBD (intrinsically base deactivated), and Ultra Cyano phases can use the basic nature of these compounds to achieve a separation that will not suffer from the problems normally resulting in peak tailing.