用奥特水性C18柱分析极性化合物的理想方法

侯先生 13691497252 Analyze Polar Compounds by Reversed Phase HPLC Using Ultra AqueousC18 Column Short-chain organic acids, amino acids, and water-soluble vitamins are examples of highly polar compounds that are difficult to retain using conventional reversed phase columns, even with little or no organic solvent in the mobile phase. The Ultra Aqueous C18 column was designed for reversed phase applications that require highly aqueous mobile phases. The analysis of six organic acids, which are difficult to retain using many conventional C18 columns, shows the Ultra Aqueous C18 column provides enhanced retention and selectivity (Figure 1). The Ultra Aqueous C18 column also gives reproducible retention times, sharper peak shapes; allows use of highly aqueous mobile phases, and may eliminate the need for sample derivatization or ion pairing reagents