采用 Agilent AssayMAP 自动进行细胞表面蛋白质分离

This study investigated the feasibility of automating an established cell surface protein isolation protocol using the Agilent AssayMAP Bravo automation platform. Epidermoid cancer cell lines - A431 was used as an in - vitro model system, and cell surface proteins were isolated using a combination of AssayMAP Bravo automation and a Pier ce Cell Surface Protein Isolation kit. The isolated proteins were then analyzed using the Agilent 2200 TapeStation system in conjunction with the Agilent P200 ScreenTape assay for differential electrophoresis profi le. The eluted proteins were then anal yzed using the Agilent HPLC - chip coupled to an Agilent 6550 iFunnel Q - TOF with dual stage ion funnel technology. Key cell surface proteins were identifi ed including extracellular matrix proteins, moderately abundant proteins including pancreatic marker protein, plectin - 1 along with F - box leucine rich repeat protein - 2, beta - actin, and PGK - 2. This study demonstrates that the AssayMAP Bravo platform can be conveniently used for automated high - throughput sample preparation workfl ows involving affi nit y purifi cations