同时使用分子相互作用仪MP-SPR、电位测定法和阻抗谱法实时监测抗体的结合

同时使用分子相互作用仪MP-SPR、电位测定法和阻抗谱法实时监测抗体的结合：抗体表征可以在各种液体，包括复杂液体，比如100%血清、尿液或唾液中进行抗体-抗原相互作用的亲和力和动力学测量。 Multi-Parametric Surface Plasmon Resonance (MP-SPR) equipped with an electrochemistry (EC) flow-cell was used to run potentiometric and impedance measurements in parallel with the MP-SPR measurement. The binding reaction of anti-methotrexate to immobilized small molecule weight drug methotrexate (MTX) was followed by MP-SPR and by potentiometry and Electrochemical Impedance Spectroscopy (EIS). Methotrexate binding produced a relatively large impedance response and antibody molecules a small response, whereas potentiometry values stayed equal during interactions. The strong binding of antibody molecules was observed in the MP-SPR response.