单细菌细胞的拉曼分析

Raman microscopy has been used to interrogate single bacteria cells – with multivariate analysis it has been shown that different species and growth phases can be quickly distinguished. Cells grown with differing amounts of 13C enriched nutrients showed Raman bands shifted to lower wavenumber due to the heavy isotope. With further multivariate analysis the cells can be classified according to the percentage uptake of 13C.