测量应用案例-2016-89

Considered a popular drug for diabetes in recent years, metformin was determined to have a moderate anti-tumor effect, particularly in breast cancer. In this study, the anticancer mechanism of metformin was verified by preparing solid lipid nanoparticles (SLNs) and chitosan-modified solid lipid nanoparticles (CSLNs) containing metformin and then estimating the potential of these SLNs for uptake in cells and mitochondria. Metformin–SLNs were prepared using an emulsification and low-temperature solidification method. The mean particle size, zeta potential, entrapment efficiency, and loading efficiency of metformin–SLNs and metformin chitosan-modified SLNs were 102.3?±?4.16 and 200.1?±?17.69?nm, –21.25?±?4.89 and 50.6?±?4.09?mv, 26.25?±?2.59% and 33.6?±?2.21%, and 1.74?±?0.16% and 1.46?±?0.10%, respectively.