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An ideal, quantitative amino acidanalysis combines speed and sensitivity with reliability of boththe derivatization reaction andthe analytical technique. Thesegoals are achieved with automated,online derivatization usingo-phthalaldehyde (OPA) for primary amino acids and 9-fluorenylmethyl chloroformate(FMOC) for secondary aminoacids; the automatedderivatization is then integratedwith rugged HPLC analysis. The complete procedure is rapid,accurate, sensitive, and reproducible using the Agilent 1100 HPLC.
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