使用液相色谱法与Q Exactive Focus HRMS联用定量检测人血浆中的类固醇

A robust and rapid LC-MS method for the determination of steroids in plasma samples using an affordable HRMS instrument has been presented and is suitable for routine research analyses. Results acquired using this method demonstrate that the Q Exactive Focus MS exhibits accuracy, precision, and sensitivity that is comparable to triple quadrupole MS instruments. Various steroid isomers can be detected in full scan but also in MS/MS acquisition showing that there is a need for an efficient chromatography. In addition, with sensitive and selective full scan data, the Q Exactive Focus HRMS can be used for more in-depth investigations when requested by researchers or biochemists.