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2.1 The reporter gene system utilizes a human cell line (101L) into which a plasmid containing a human CYP1A1 promoter and 5'-flanking sequences fused to a reporter gene, firefly luciferase, have been stably integrated. In the presence of CYP1A1-inducing compounds, the enzyme luciferase is produced, and its reaction with luciferin can be detected by measuring relative light units (RLUs) in a luminometer. CYP1A1-inducing environmental contaminants include polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), coplanar polychlorinated biphenyl congeners (PCBs), and high molecular weight polynuclear aromatic hydrocarbons (PAHs).
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