锤子
消耗积分 : 10积分 移动终端:免积分
2.1 Test kits are commercially available for this method. The manufacturer's directions should be followed. 2.2 In general, the method is performed using an extract of a solid sample. Solid samples are prepared by extraction with a mixture of hydrochloric and nitric acids for ten minutes and then buffered prior to analysis. The sample is added to a tube (treated with BSA-glutathione) and incubated at ambient temperatures for five minutes. The mercuric ions bound to the sulflhydryl groups of the BSA-glutathione are now reacted with a reconstituted antibody specific for mercury and incubated for five more minutes. A peroxidase conjugate is added to the sample, reacting with any mercury specific antibody. The substrate is then added forming a color that is in proportion to the amount of mercury originally present in the sample. The color produced is then spectrophotometrically compared with the control standards.
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