TRIAZINE HERBICIDES AS ATRAZINE IN WATER

2.1 An accurately measured volume of sample (as little as 200 µL for some testing products) is mixed with a volume of enzyme-atrazine conjugate reagent in a test tube or a microtiter plate that has an anti-atrazine antibody immobilized on the surface, or in a vessel to which particles (magnetic particles for one testing product) with an immobilized antibody on the surface are added. The conjugate "competes" with the atrazine present in the sample for binding to the immobilized anti-atrazine antibody. The mixture is incubated at the temperature, and for the time, described in the manufacturer's instructions. (Testing products may employ other solid-phase support configurations, or even eliminate the solid-phase support. The summary here is intended to be generic and not to limit the development of other testing products).