Nanobrook Omni测量应用案例-13

We suggest a new strategy for obtaining fluorescent fingerprints of samples for their recognition using chemometric treatment. The technique is based on the use of a blend of fluorophores of various nature emitting over a wide range of spectrum and changing the emission intensity to a different extent in the presence of different analytes. In this feasibility study we prepared a blend of up to 5 fluorophores covering the emission range of 360–800 nm: quantum dots CdSe/CdS/ZnS and PbS, a Schiff base produced from o-phthalic dialdehyde and polyethyleneimine, and fluorescein and rhodamine B attached to silica nanoparticles. We showed that the attachment of the fluorescent dyes to a nanoadsorbent appreciably increased their responsiveness to quenching/dequenching by organic compounds. As model analytes, we used amikacin, sulfamethoxazole, piracetam and chloramphenicol and their mixtures in equal concentrations.