在制备型液相色谱中自动生成聚焦梯度曲线——使用Agilent 1260 Infinity 自动化液质联用纯化系统进行样

With the Agilent 1260 Infinity automated LC/MS purifi cation system, the purification process has reached a new level of automation. A tailored-gradient profile for each target compound was generated to increase the resolution, increase columnload, and reduce the cycle runtime. The Agilent automated purifi cation software automatically generated the different tailored-gradient profi les. This Technical Overview describes an automated purifi cation process of a compound mixture, and shows cross-platform compatibility between different analytical LC systems and the Agilent 1260 Infi nity automated LC/MS purifi cation system. A standard mix was analyzed using four different analytical (U)HPLC systems, using different gradient profi les and different column dimensions. The four analytical result fi les were used to calculate gradient profi les, and purify target compounds. Through the use of this intelligent software algorithm, we obtained automated tailored-gradient profi les for all four samples using the purifi cation process. We purified 100 mg of a sample mix containing 24 mg of the target compound. We obtained 90 % recovery and 99 % purity of the target compound after purifi cation.