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The purification of a valuable product or product library after a long and complex synthesis is a very important process. The Agilent Automated Purifi cation Software introduces an Open-Access-based solution for isolating substances, with automated focused gradient to simplify the pre- and post-purification steps. This Technical Overview describes the easy steps to follow, from crude sample analytical scouting submission to pure target product archiving or screening. For example, a purification sample containing a target peak overlapped by an impurity was purifi ed on the Agilent 1260 Infinity Preparative-Scale LC/MS Purifi cation System using the Automated Purifi cation Software. This purifi cation produced a pure target compound with 88 % recovery, saving 40 % of solvent and runtime compared to a standard purifi cation method.
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