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Ultra high performance liquid chromatography (UHPLC) and LC/MS/MS methods determine free carbonyl compounds in air after derivatization with 2,4-dinitrophenylhydrazine. The methods use UHPLC with DAD detection and triple quadrupole LC/MS/MS to identify and quantitate the target analytes. Using an Agilent InfinityLab Poroshell 120 EC-C18 HPLC column, UHPLC separates 14 carbonyl-DNPH components in 12 minutes. LC/MS/MS gives a lower limit of detection than LC alone. Using multiple reaction monitoring, more carbonyl-DNPH can be resolved.
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