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A novel imidazolium ionic liquid mixed-mode stationary phase (named IBON) was successfully prepared by a controlled thiol-initiated radical polymerization method. The successful preparation of the IBON stationary phase was certified by Fourier transform infrared (FT-IR) spectrometry, elemental analysis, scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). Meanwhile, the zeta potential showed the surface potential of the IBON stationary phase, while the results of gel permeation chromatography (GPC) demonstrated the successful control of the surface-initiated polymerization. The retention behaviour was investigated by various types of analytes, including acidic compounds, basic compounds and neutral compounds (containing polar nucleosides and bases, non-polar benzene series).
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