反相色谱分析、纯化多肽和蛋白

The Handbook of Analysis and Purification of Peptides and Proteins by Reversed-Phase HPLC Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 Mechanism of Interaction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 The Role of the Column in Polypeptide Separations . . . . . . . . . . . . . . . . . . . .8 Analytical Conditions: The Role of the Mobile Phase and Temperature . . . .17 Reversed-Phase HPLC/Mass Spectrometry . . . . . . . . . . . . . . . . . . . . . . . . . . .26 The Role of Reversed-Phase HPLC in Proteomic Analysis . . . . . . . . . . . . . .30 Examples of the Use of Reversed-Phase HPLC in the Analysis of Polypeptides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32 HPLC as a Tool to Purify and Isolate Polypeptides . . . . . . . . . . . . . . . . . . . .40 Viral Inactivation During Reversed-Phase HPLC Purification . . . . . . . . . . . .50 Appendices Appendix A: Column Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 Appendix B: The Care and Maintenance of Reversed-Phase Columns . . . . .53 Appendix C: The Effect of Surfactants on Reversed-Phase Separations . . . .56 Appendix D: Ion Exchange Chromatography: Orthogonal Analytical Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58 Appendix E: The Effect of System Hardware on Reversed-Phase HPLC Polypeptide Separations . . . . . . . . . . . . . . . . . . . .60 Technical References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62