epMotion 高效完成定量PCR体系构建工作完全无交叉污染.

This application note shows that the epMotion family of automated liquid handling systems is ideal for reliable and reproducible setup of sensitive real-time quantitative PCR (qPCR) assays in daily routine, without the risk of cross contamination. The complete workflow from Master Mix preparation to 96-well PCR plate setup was done with the automation system. Cross contamination assay in chess board pattern was performed according to FDA guidance. Runs on two independent epMotion systems with 2 plates each on 3 days are compared in this document. The assay reproducibility was excellent with low coefficients of variation for both intra and inter-plate comparisons of Ct values. Automated assay setups were carried with the epMotion 5073 and the 5075 systems.