资料摘要
资料下载Severe acute respiratory syndrome (SARS) coronavirus is a novel human coronavirus and is responsible for SARS infection. SARS coronavirus 3C-like proteinase (SARS 3CLpro) plays key roles in viral replication and transcription and is an attractive target for anti-SARS drug discovery. In this report, we quantitatively characterized the dimerization features of the full-length and N-terminal residues 1–7 deleted SARS 3CLpros by using glutaraldehyde cross-linking SDS-PAGE, size-exclusion chromatography, and isothermal titration calorimeter techniques. Glutaraldehyde cross-linking SDSPAGE and size-exclusion chromatography results show that, similar to the full-length SARS 3CLpro, the N-terminal deleted SARS 3CLpro still remains a dimer/monomer mixture within a wide range of protein concentrations. Isothermal titration calorimeter determinations indicate that the equilibrium dissociation constant (Kd) of the N-terminal deleted proteinase dimer (262 M) is very similar to that of the full-length proteinase dimer (227 M). Enzymatic activity assay using the fluorescence resonance energy transfer method reveals that N-terminal deletion results in almost complete loss of enzymatic activity for SARS 3CLpro. Molecular dynamics and docking simulations demonstrate the N-terminal deleted proteinase dimer adopts a state different from that of the full-length proteinase dimer, which increases the angle between the two protomers and reduces the binding pocket that is not beneficial to the substrate binding. This conclusion is verified by the surface plasmon resonance biosensor determination, indicating that the model substrate cannot bind to the N-terminal deleted proteinase. These results suggest the N terminus is not indispensable for the proteinase dimerization but may fix the dimer at the active state and is therefore vital to enzymatic activity.
微库仑法测定废水中的可吸附有机卤素(AOX)和纸浆中的总有机卤素(TOX)
简介:纸浆和纸品工艺过程中用到高浓度的化学物,如氯,二氧化氯和盐酸用来漂白,由此产生的废水包含有机氯化物,这些物质对环境有害,因此废水中可吸附有机卤素AOX和纸浆中的总有机卤素TOX显得尤为重要。
应用指南第一篇:从等温吸附线中可以获得什么信息?
简介:比表面和孔径测量: Microtrac MRB采用气体吸附法为粉体样品的比表面值,BET值和孔径测量提供了吸附系列的分析仪器。开发和生产以及装配地点位于日本大阪的表面分析中心。 我们为客户提供先进的技术,以不断获得可靠的结果。创新和质量是我们成功的基础。
应用指南3:通过BET方法分析活性炭的比表面积(I型等温吸脱附曲线)
简介:比表面和孔径测量: Microtrac MRB采用气体吸附法为粉体样品的比表面值,BET值和孔径测量提供了吸附系列的分析仪器。开发和生产以及装配地点位于日本大阪的表面分析中心。 我们为客户提供先进的技术,以不断获得可靠的结果。创新和质量是我们成功的基础。
R1_No.2_ENG_TheevaluationoftheSpecificsurfaceareaofporousSilicabyBETmethod
简介:比表面和孔径测量: Microtrac MRB采用气体吸附法为粉体样品的比表面值,BET值和孔径测量提供了吸附系列的分析仪器。开发和生产以及装配地点位于日本大阪的表面分析中心。 我们为客户提供先进的技术,以不断获得可靠的结果。创新和质量是我们成功的基础。
R1_No.3_ENG_TheevaluationoftheSpecificsurfaceareaofactivecarbon(TypeIadsorptionisotherm)byBETmethod
简介:比表面和孔径测量: Microtrac MRB采用气体吸附法为粉体样品的比表面值,BET值和孔径测量提供了吸附系列的分析仪器。开发和生产以及装配地点位于日本大阪的表面分析中心。 我们为客户提供先进的技术,以不断获得可靠的结果。创新和质量是我们成功的基础。
相关产品
麦奇克激光粒度仪 S3500系列
Rudolph AUTOPOL VI高精度旋光仪
Rudolph数字式密度计DDM 2911
全自动氨基酸分析仪 Biochrom 30+
纳米颗粒跟踪分析仪(生物纳米追踪)
流式影像仪 FlowCam®8400(cyano)
荷兰TE硫氮分析仪 XPLORER-V系列
自动液体处理系统
TURBISCAN 稳定性分析仪(多重光散射仪)
TURBISCAN 稳定性分析仪(多重光散射仪)
全自动比表面分析仪 (动态/静态法)
荷兰TE 总氯分析仪XPLORER TX
荷兰TE 总硫分析仪XPLORER TS
荷兰TE 总有机卤素分析仪XPLORER AOX
荷兰TE 总硫总氮分析仪XPLORER NS
关注
拨打电话
留言咨询