资料摘要
资料下载It was found that native amino acids enhanced the chemiluminescence (CL) reaction between luminol and BrO2 in an alkaline aqueous solution. This has led to the development of a facile and highly sensitive CL detection scheme for the determination of amino acids in biological samples after capillary electrophoretic (CE) separation. The CE-CL conditions were optimized. An electrophoretic buffer of 2.5 6 1022 M sodium borate (pH 9.4) containing 1 6 1024 M luminol was used. The oxidizer solution of 8*10-4 M NaBrO in 0.1 M sodium carbonate buffer solution (pH 12.5) was introduced postcolumn. Under the optimal conditions, the detection limits were 1.0 6 1027 M for glutamic acid (Glu) and 1.361027 M (S/N = 3) for aspartic acid (Asp). The relative standard deviations (RSDs) of peak area and migration time were in the ranges of 3.8–4.3% and 1.4–1.6%, respectively. The present method was applied to the determination of excitatory amino acids (i.e., Asp and Glu) in rat brain tissue and monkey plasma. The levels of these major excitatory amino acids in monkey plasma were quantified for the first time and found to be 1.17 6 0.1761025 M (mean 6 SD, n = 6) for Glu and 1.64 6 0.19 6 1026 M for Asp, which were comparable with the levels in human plasma.
文献贡献者
In Situ Synthesis of MIL-100(Fe) in the Capillary Column for Capillary Electrochromatographic Separation of Small Organic Molecules
简介：Because of the unusual properties of the structure, the metal organic frameworks (MOFs) have received great interest in separation science. However, the most existing methods for the applications of MOFs in separation science require an off-line procedure to prepare the materials. Here, we report an in situ, layerby- layer self-assembly approach to fabricate MIL-100(Fe) coated open tubular (OT) capillary columns for capillary electrochromatography. By a controllable manner, the OT capillary columns with a tailored MIL-100(Fe) coating have been successfully synthesized. The results of SEM, XRD, FT-IR, and ICP-AES indicated that MIL- 100(Fe) was successfully grafted on the inner wall of the capillary. Some neutral, acidic and basic analytes were used to evaluate the performance of the MIL-100(Fe) coating OT capillary column. Because of the size selectivity of lattice aperture and hydrophobicity of the organic ligands, three types of analytes were well separated with this novel MIL-100(Fe) coating OT capillary column. For three consecutive runs, the intraday relative standard deviations (RSDs) of migration time and peak areas were 0.4?4.6% and 1.2?6.6%, respectively. The interday RSDs of migration time and peak areas were 0.6?8.0% and 2.2?9.5%, respectively. The column-to-column reproducibility of retention time was in range of 0.6?9.2%. Additionally, the 10 cycles OT capillary column (10-LC) could be used for more than 150 runs with no observable changes on the separation efficiency.
CL3030毛细管电泳液相色谱一体机使用说明书
简介：CL3030高效毛细管电泳液相色谱一体机既具有高效液相色谱功能，也有高效毛细管电泳仪的功能。您只需要在紫外检测器上改装液相色谱流通池就可以进行液相色谱的分析，更换毛细管电泳检测池即可进行毛细管电泳的试验。紫外检测器部分采用国产检测器，高压电源和高压输液泵均采用进口原件组装。CL3030高效毛细管电泳液相色谱一体机的设计不仅可以节省经费，还节省实验室空间，更提高了仪器的使用效率。本仪器可广泛应用于环境保护、生物化学、医学卫生 、临床检测、食品卫生、药品检测、农业化学等领域的生产、教学和科学研究。特别适合于各种科研机构和大专院校开展研究和教学实验。
CL1040全自动毛细管电泳仪使用说明书
简介：CL1040全自动毛细管电泳仪系统具有高效、快速、经济、进样量小、灵敏度高等优点，可广泛应用于环境保护、生物化学、医学卫生 、临床检测、食品卫生、药品检测、农业化学等领域的生产、教学和科学研究。它是基于被分离物质的净电荷与质量比的差异，各组分按其表面电荷的差异，以不同的速度在毛细管内的电解质溶液中移动而导致分离，特别适合于各种科研机构和大专院校开展研究和教学实验。
CL1030高效毛细管电泳仪使用说明书
简介：CL1030高效毛细管电泳仪系统具有高效、快速、经济、进样量小、灵敏度高等优点，可广泛应用于环境保护、生物化学、医学卫生 、临床检测、食品卫生、药品检测、农业化学等领域的生产、教学和科学研究。特别适合于各种科研机构和大专院校开展研究和教学实验。
CL1010毛细管电泳仪（安培检测器）使用说明书
简介：CL1010高效毛细管电泳仪系统具有高效、快速、经济、进样量小、灵敏度高等优点，可广泛应用于环境保护、生物化学、医学卫生 、临床检测、食品卫生、药品检测、农业化学等领域的生产、教学和科学研究。特别适合于各种科研机构和大专院校开展研究和教学实验。
相关产品
关注
拨打电话
留言咨询
