全血/血清/血浆null检测 | 快速测定动物血浆中的治疗性抗体-赛默飞色谱与质谱

方案摘要

方案下载

应用领域	医疗/卫生
检测样本	全血/血清/血浆
检测项目
参考标准	SMART Digest IA USERS MANUAL: https://tools.thermofisher.com/content/sfs/manuals/Man-21549-SMART-Digest-IA-User-Man21549-EN.pdf

• The rapid processing of antibodies using the SMART Digest IA kits allows development of faster methods compared to conventional protein enrichment and digestion by simplifying sample preparation and enabling a reproducible, sensitive, and fully integrated LC/MS/MS workflow. • This application shows a rapid, simple, and sensitive method for the quantification of human IgG in mouse plasma using the SMART Digest IA kits with a total time of 3–4 hours that compares to a process that can take up to 24 hours. • The method described provides a significant simplification for the affinity proteomics process within a single well providing increased sensitivity and reduced sample preparation time. • This Application Note provides a general approach for quantifying low abundance mAbs with reproducible results across a broad dynamic range for all three types of SMART Digest (IA) kits (SMART digest IA Streptavidin, Protein A, and Protein G).

方案下载

方案详情

To demonstrate the rapid quantitation of a low-level human IgG in animal
plasma by LC/MS/MS using the Thermo Scientific™ SMART Digest™
Immunoaffinity (IA) kits (including magnetic and non-magnetic versions of the
Streptavidin kit, Protein A kit, and Protein G kit), which combine the IA capture
and digestion process into a single well. The assay is required to be both
selective and accurate.

• The rapid processing of antibodies using the SMART
Digest IA kits allows development of faster methods
compared to conventional protein enrichment and
digestion by simplifying sample preparation and
enabling a reproducible, sensitive, and fully integrated
LC/MS/MS workflow.

• This application shows a rapid, simple, and sensitive
method for the quantification of human IgG in mouse
plasma using the SMART Digest IA kits with a total time
of 3–4 hours that compares to a process that can take
up to 24 hours.

• The method described provides a significant
simplification for the affinity proteomics process within
a single well providing increased sensitivity and
reduced sample preparation time.

• This Application Note provides a general approach for
quantifying low abundance mAbs with reproducible
results across a broad dynamic range for all three types
of SMART Digest (IA) kits (SMART digest IA
Streptavidin, Protein A, and Protein G).

上一篇高压离子色谱Inuvion快速测定自来水中的常见阴离子

下一篇使用LC-MS MS检测人血浆中的17种抗癫痫药及其代谢产物

文献贡献者

赛默飞色谱与质谱

钻石会员丨第23年

查看全部资料