方案摘要
方案下载应用领域 | 食品/农产品 |
检测样本 | 茶饮料 |
检测项目 | 食品添加剂>山梨酸, 咖啡因, 苯甲酸 |
参考标准 | 无 |
毛细管电泳法用于测定饮料中咖啡因、抗坏血酸及其盐类、山梨酸及其盐、苯甲酸及其盐类、乙酰磺胺酸钾、糖精及其盐类的质量浓度。该法可适用于所有类型的非酒精和酒精饮料,包括运动和烈性饮料,果汁,啤酒和啤酒产品,葡萄酒,白兰地和烈酒,伏特加等。
The method1 is used for the determination of the mass concentration of caffeine, ascorbic acid and its salts, sorbic acid and its salts, benzoic acid, and its salts, acesulfame K, saccharin and its salts in beverages.
MEASUREMENT METHOD
The micellar electrokinetic chromatography (MEKC) allows separation of neutral and ionic forms of analyzed components. The components are detected by intrinsic absorption at a wavelength of 254 nm.
Measurement ranges of analyzed components are presented in the table below.
Compound | CSFA code | Е number | Determined form | Measurement range*, mg/L |
Caffeine | – | – | Caffeine |
10–1000 |
Sorbic acid | INS 200 | E 200 |
Sorbic acid | |
Sodium sorbate | INS 201 | E 201 | ||
Potassium sorbate | INS 202 | E 202 | ||
Calcium sorbate | INS 203 | E 203 | ||
Benzoic acid | INS 210 | E 210 | ||
Sodium benzoate Potassium benzoate | INS 211 INS 212 | E 211 E 212 | Benzoic acid | |
Calcium benzoate | INS 213 | E 213 | ||
Ascorbic acid | INS 300 | E 300 |
Ascorbic acid | |
Sodium ascorbate | INS 301 | E 301 | ||
Calcium ascorbate | INS 302 | E 302 | ||
Potassium ascorbate | INS 303 | E 303 | ||
Acesulfame K | INS 950 | E 950 | Acesulfame K | |
Saccharin | INS 954(i) | E 954(i) |
Sodium saccharin | |
Calcium saccharin | INS 954(ii) | E 954(ii) | ||
Potassium saccharin | INS 954(iii) | E 954(iii) | ||
Sodium saccharin | INS 954(iv) | E 954(iv) |
* For every mentioned form of the food additive.
The present method does not allow separation of such food additives as E 200 – E 203, E210 – E 213, E 300 – E 303, and E 954. Other sweeteners (aspartame, cyclamate) synthetic food dyes, vitamins B, and vanillin do not hinder the analysis if added in concentrations, typical for the analyzed drinks.
The CAPEL capillary electrophoresis system is used in measurements. Data acquisition, collection, processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.
Lumex Instruments complex set, order No 0300002054.
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