软饮料中咖啡因、抗坏血酸、防腐剂和人工甜味剂的测定（LUMEX毛细管电泳法）

2018/07/24   下载量： 1 次

方案摘要

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应用领域 食品/农产品
检测样本 茶饮料
检测项目 食品添加剂>山梨酸, 咖啡因, 苯甲酸
参考标准 无

毛细管电泳法用于测定饮料中咖啡因、抗坏血酸及其盐类、山梨酸及其盐、苯甲酸及其盐类、乙酰磺胺酸钾、糖精及其盐类的质量浓度。该法可适用于所有类型的非酒精和酒精饮料，包括运动和烈性饮料，果汁，啤酒和啤酒产品，葡萄酒，白兰地和烈酒，伏特加等。

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INTRODUCTION

The method1 is used for the determination of the mass concentration of caffeine, ascorbic acid and its salts, sorbic acid and its salts, benzoic acid, and its salts, acesulfame K, saccharin and its salts in beverages.

The method can be applied for all types of non-alcoholic and alcoholic beverages including sport and energetic drinks, juices, beer and beer products, wines, brandy and spirits, liquors, and vodka.

MEASUREMENT METHOD

The micellar electrokinetic chromatography (MEKC) allows separation of neutral and ionic forms of analyzed components. The components are detected by intrinsic absorption at a wavelength of 254 nm.

MEASUREMENT RANGE

Measurement ranges of analyzed components are presented in the table below.

 

Compound

CSFA code

Е number

Determined form

Measurement range*, mg/L

Caffeine

–

–

Caffeine

 

 

 

 

 

 

 

 

 

10–1000

Sorbic acid

INS 200

E 200

 

 

Sorbic acid

Sodium sorbate

INS 201

E 201

Potassium sorbate

INS 202

E 202

Calcium sorbate

INS 203

E 203

Benzoic acid

INS 210

E 210


Sodium benzoate

Potassium benzoate

INS 211

INS 212

E 211

E 212

Benzoic acid

Calcium benzoate

INS 213

E 213


Ascorbic acid

INS 300

E 300

 

 

Ascorbic acid

Sodium ascorbate

INS 301

E 301

Calcium ascorbate

INS 302

E 302

Potassium ascorbate

INS 303

E 303

Acesulfame K

INS 950

E 950

Acesulfame K

Saccharin

INS 954(i)

E 954(i)

 

 

Sodium saccharin

Calcium saccharin

INS 954(ii)

E 954(ii)

Potassium saccharin

INS 954(iii)

E 954(iii)

Sodium saccharin

INS 954(iv)

E 954(iv)

* For every mentioned form of the food additive.

The present method does  not allow separation of such food additives as E 200 – E 203, E210 – E 213,         E 300 – E 303, and E 954. Other sweeteners (aspartame, cyclamate) synthetic food dyes, vitamins B, and vanillin do not hinder the analysis if added in concentrations, typical for the analyzed drinks.

EQUIPMENT AND REAGENTS

The CAPEL capillary electrophoresis system is used in measurements. Data  acquisition,  collection,  processing and output are performed using a personal computer running under WINDOWS® XP/7/8/10 operating system with installed dedicated software package ELFORUN.

Lumex Instruments complex set, order No 0300002054.


上一篇 CAPEL毛细管电泳测定大麻及大麻原料中14种大麻素
下一篇 食品添加剂和饲料添加剂中抗坏血酸及其盐类的测定（LUMEX毛细管电泳法）

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LUMEX INSTRUMENTS (鲁美科思分析仪器)
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