方案摘要
方案下载| 应用领域 | 农/林/牧/渔 |
| 检测样本 | 饲料 |
| 检测项目 | 营养成分>氨基酸 |
| 参考标准 | GB/T 19371.2-2007 饲料中蛋氨酸羟基类似物的测定高效液相色谱法 |
毛细管电泳(CE)技术具有分离效率高、分析速度快、受基质干扰小、试剂样品消耗少且对环境友好等优点。采用毛细管电泳法可以测定饲料添加剂中的蛋氨酸羟基类似物，该法的检测波长为200nm。
The method is used for the determination of the mass fraction of methionine hydroxy analogue (DL-2-hydroxy-4-(methylthio)butyric acid (HMTBa) and its calcium salt) in fodder additives by capillary electrophoresis.
Currently, fodder additives containing HMTBa or its calcium salt, are presented on the market under such brand names as MHA®, Alimet®, Rhodimet®, and others.
For the determination of free methionine in fodder additives use the method М 04-63-2016 (Lumex Instruments set, order No 0300002780).
The measurement method is based on capillary zone electrophoresis with direct UV detection at the wavelength of 200 nm.
The measurement range for HMTBa is 60–100%. If necessary, a result of analysis may be expressed as methionine or calcium salt of HMTBa.
CE method for the determination of HMTBa has a lot of advantages compared with other analytical methods used for this purpose, such as HPLC, potentiometry, complexometry:
· The HPLC column is not required, so the cost of the analysis drops dramatically compared with HPLC.
· Direct determination of the main analyte (HMTBa) in contrast to the indirect determination of calcium (complexometry) and/ or total organic sulfur (potentiometry) content.
· Analysis time using CAPEL CE system is less than 5 minutes.
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