使用 SEC 和质谱分析抗体药物偶联物

2016/07/21   下载量： 11 次

方案摘要

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应用领域 制药/生物制药
检测样本 治疗类生物药品
检测项目
参考标准 -

Recombinant monoclonal antibodies (mAbs) have emerged as important therapeutic agents for the treatment of different diseases. This technology has been extended by the development of antibody drug conjugates (ADCs). ADCs are mAbs to which cytotoxic payloads are covalently attached, enabling the delivery of potent drugs to a specific target. This Application Note describes the analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS) using the Agilent 1290 Infinity II LC system with an Agilent 6530 Accurate Mass Q-TOF LC/MS system. We demonstrate that SEC is a straightforward way to introduce mAbs and ADCs into the LC/MS system, requiring little method optimization. From the MS data collected for the ADC, it was possible to calculate a drug-to-antibody ratio (DAR) of 3:2.

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上一篇 使用 Agilent 8890 气相色谱和 TCD/FID 系统分析氢气中的氦气、 氩气、氮气和烃类杂质
下一篇 利用 1290 Infinity II 液相色谱测定啤酒中的苦味和类似丁香的味道

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安捷伦科技(中国)有限公司
钻石会员 丨 第23年
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