其他null检测 | 吡咯里西啶生物碱的精确质量 Q-TOF LC/MS 及全离子 MS/MS-安捷伦科技(中国)有限公司

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应用领域	制药
检测样本	其他
检测项目
参考标准	-

Pyrrolizidine alkaloids (PAs) and their N - oxides (PANOs) have serious hepatotoxic effects in both humans and animals, necessitating a method capable of detecting, characterizing, and quantifying them using a robust assay. While triple quadrupole LC/MS systems are ideal for targeted detection, targeted analysis requires standards that are not always easily obtained. Only a fraction of known naturally occurring PAs are available as standards. Triple quadrupole analyses are also not useful for screening unknowns. High - res olution mass spectrometers that provide accurate - mass data enable characterization of unknown PAs/PANOs without the need for specific standards. This application note presents a workflow that uses accurate - mass Q - TOF LC/MS, with All Ions MS/MS and databas e/mass spectral library searching, for the selective and sensitive characterization, screening, tentative identification, and quantification of PAs and PANOs in botanicals and dietary supplements. Twenty - five PA/PANO standards were characterized using their high - resolution accurate - mass MS/MS spectra. These data were used to create a personal com - pound database and library (PCDL) that was then used to screen for PAs/PANOs in 44 botanical and dietary supplement samples. Quantitation was performed using Q - TOF LC/MS response. PAs/PANOs not specifically targeted were tentatively identified using characteristic fragment ions and fragmentation patterns. The PAs/PANOs characterized could potentially lead to the discovery of more unique PAs in additional botan ical and dietary supplement sample s.

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上一篇使用 Agilent 5800 ICP-OES 测定固态电解质锂镧锆钽氧 (LLZTO) 中的主量元素

下一篇使用 Agilent 1290 Infinity II 二维液相色谱解决方案对茶进行全二维液相色谱分析

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安捷伦科技(中国)有限公司

钻石会员丨第23年

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