其他食品过敏原>其他检测 | 多过敏原检测：测定独特的坚果及树生坚果多肽标记物-安捷伦科技(中国)有限公司

方案摘要

方案下载

应用领域	食品/农产品
检测样本	其他食品
检测项目	过敏原>其他
参考标准	-

The presence of hidden nut and tree nut allergens in foods can result in serious health issues, necessitating a method capable of detecting and quantifying them at trace levels using a single robust assay. To find specific peptide markers that can be used to determine the presence or absence of specific nuts in food, proteins unique to 11 tree nuts (almond, pecan, cashew, walnut, hazelnut, pine nut, brazil nut, macadamia nut, pine nut, chestnut, and coconut) and peanut were enzymatically digested and analyzed using accurate - mass Q - TOF LC/MS. Each marker peptide was selected by establish ing its presence in raw and roasted nuts, processed and unprocessed food, abundance (sensitivity), sequence size, and uniqueness to a specific nut. The National Center for Biotechnology Information (NCBI) nr database searches were performed to confirm pep tide identities, and to ensure that the marker peptides chosen were not present in other nuts or common food ingredients such as barley, corn, rice, soy, and wheat. Two marker peptides were selected for each tree nut, and four were selected for peanuts. An alysis of peptide digests from grains such as barley, corn, rice, quinoa, soy, and wheat did not present interfer - ences. The peptide markers were tested to determine if they could be used to screen common foods for the presence of the 11 tree nuts and pea nut at sub - ppm levels. Foods containing nuts as listed on the label showed a response for the cor - rect nut. Foods processed using equipment also used to process other tree nuts or peanuts were in certain cases found to contain these other nuts.

方案下载

上一篇使用 Agilent 5800 ICP-OES 测定固态电解质锂镧锆钽氧 (LLZTO) 中的主量元素

下一篇使用多中心切割二维液相色谱进行杂质分析的方法开发

文献贡献者

安捷伦科技(中国)有限公司

钻石会员丨第23年

查看全部资料