方案摘要
方案下载| 应用领域 | 生物产业 |
| 检测样本 | 其他 |
| 检测项目 | |
| 参考标准 | 暂无 |
?This Application Note demonstrates: • Configuration of the Agilent 1200 Series Rapid Resolution LC (RRLC)analytical scale (AS) purification system. • Use of the Agilent 1200 Series RRLC AS purification system with anoptimized method for purification of peptide nucleic acids from solidphase synthesis.
This Application Note demonstrates:
• Configuration of the Agilent 1200 Series Rapid Resolution LC (RRLC)analytical scale (AS) purification system.
• Use of the Agilent 1200 Series RRLC AS purification system with anoptimized method for purification of peptide nucleic acids from solidphase synthesis.
This Application Note demonstrates the use of the Agilent 1200 Series Rapid Resolution LC analytical scale purification system for the purification of PNAs from solid-phase synthesis. For longer PNAs (~20-mer), an easier and faster purification is achieved by purifying the “Fmoc-on” product. The same HPLC method can also be used for dye-labeled PNAs. The desired products can be isolated from the crude mixture in high purity by an automated purification process that is triggered by a UV signal. The purity of the isolated PNA can be confirmed by reanalysis of the fraction.
The Agilent 1200 Series RRLC allows for fully automated purification of Fmoc-protected and fluorescence-labeled PNAs within 4 minutes, including washing and equilibration of the column. With an additional alternating column regeneration, purification of a PNA will only take 2.5 minutes per sample. As the spectra indicate, even shorter purification times can be established by using steeper gradients, thereby diminishing the overall purification time for high-throughput applications.
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