方案摘要
方案下载| 应用领域 | 制药/生物制药 |
| 检测样本 | 其他 |
| 检测项目 | 其他 |
| 参考标准 | 暂无 |
On the Agilent OpenLAB CDS, fraction collection can be performed with a UV detector, or any additional detector, such as an Evaporative Light Scattering Detector (ELSD). In another Technical Overview1 , we described a simple way to split the preparative fl ow on the Agilent preparative LC systems to trigger the collection with an ELSD. This Technical Overview shows how to optimize peak-based fraction collection triggered by an ELSD. We describe the system setup, the delay calibration between detector and fraction collector, and the use of the variable light sensitivity of the Agilent 1260 Infi nity Evaporative Light Scattering Detector. We also discuss the peak-based fraction collection settings required to achieve the best purity and recovery.
Abstract
On the Agilent OpenLAB CDS, fraction collection can be performed with a UV detector, or any additional detector, such as an Evaporative Light Scattering Detector (ELSD). In another Technical Overview1 , we described a simple way to split the preparative fl ow on the Agilent preparative LC systems to trigger the collection with an ELSD.
This Technical Overview shows how to optimize peak-based fraction collection triggered by an ELSD. We describe the system setup, the delay calibration between detector and fraction collector, and the use of the variable light sensitivity of the Agilent 1260 Infi nity Evaporative Light Scattering Detector. We also discuss the peak-based fraction collection settings required to achieve the best purity and recovery.
Introduction
The evaporative light scattering detector (ELSD) is commonly used to detect compounds without a chromophore. Therefore, it is ideal for the purifi cation of amino acids, peptides, carbohydrates, and lipids. Using the Agilent Splitter Kits corresponding to the required purifi cation scale on the Agilent Purifi cation System1 , the signal from the ELSD can be used for peak-based collection of the target compounds. To ensure the best reliability and purity of the collected fractions, several parameters need to be optimized.
The delay time between the detector and the fraction collector needs to be measured. This delay is the difference between the time the compound is monitored in the detector, and the time when this compound reaches the diverting valve of the fraction collector. Correct measurement of this delay time is an important step of the system optimization. This procedure is automated on the Agilent 1260 Infinity Fraction Collectors using Agilent Lab Advisor Software.
This Technical Overview also describes how to improve parameters such as the light intensity of the ELSD, and the fraction collector settings.
Conclusion
This Technical Overview shows how to optimize fraction collection with the Agilent 1260 Infi nity Evaporative Light Scattering Detector. The delay calibration performed with the Lab Advisor Software provides a simple and quick method to calibrate the delay volume between detector and fraction collection. With the possibility of light intensity adjustment, the 1260 Infi nity ELSD offers a fl exible solution for preparative liquid chromatography by increasing or decreasing the sensitivity of the detector with respect to the sample concentration. These features ensure best reliability of the purifi cation results.
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