方案摘要
方案下载应用领域 | |
检测样本 | |
检测项目 | |
参考标准 |
Frankia are gram-positive, filamentous, nitrogen-fixing actinobacteria that are symbiotic with over 200 different species of plants. Frankia produce three cell types: vegetative hyphae, spores located in sporangia, and the unique lipid-enveloped cellular structures, termed vesicles. Vesicles are formed inside plant cell nodules, or in culture under nitrogen limiting conditions, and act as specialized structures for the nitrogen fixation process. The mature vesicle is surrounded by an envelope that extends down the stalk of the vesicle past the basal septum, which separates the vesicle from the hyphae. Techniques have been developed for the isolation and urification of intact vesicles from Frankia grown in culture [1,2,3]. Initial investigations on the properties of purified vesicles have focused on nitrogen metabolism [3,4]. Protein extraction from vesicles has historically been difficult and a significant bottleneck to proteomic studies because vesicles are resilient structures that are difficult to disrupt. Reliable and comprehensive proteomic maps of Frankia hyphae and vesicles can only be assured when proteins are isolated reproducibly and in a manner in which they are accurately represented in the downstream analysis. For example, 2DGE can be an accurate representation of a proteome only if the entire protein constituency of cells is recovered during the sample preparation process. While French press treatment effectively lyses hyphae, it fails to disrupt Frankia vesicles. Pressure Cycling Technology (PCT) used in combination with a specialized lysis reagent effectively disrupted purified vesicles enabling further investigation of the proteome of the vesicles.
【CEM】电子设备组件样品制备用于IEC 62321-7-2:2017方法六价铬含量分析
【eralytics】结合蒸气压与密度的测量
【CEM】环状二硫键桥肽的全自动合成
相关产品
CEM Mars6 高通量密闭高压微波消解仪
EDGE全自动快速溶剂萃取仪
CEM MARS-X 微波溶剂萃取系统
ISCO X系列高压高精度柱塞泵
Hanson 6/8杯药物溶出度仪
ISCO 快速制备液相色谱仪
Katanax X600重型电熔样机/熔融炉/熔片机
CEM Smart 6 通用微波水分测量仪
Hanson 14杯药物溶出度仪
CEM Discover 2.0 300mL腔体环形聚焦单模微波合成仪
SPEX大容量液氮冷冻研磨仪
CEM ORACLE通用快速脂肪分析仪
HG-600 Geno/Grinder (原SPEX 2010)高通量动植物组织研磨机
SPEX BM-400/450高能量球磨机(原SPEX 8000M/D)
SPEX CG-500高通量液氮冷冻研磨仪(SPEX 6875D)
关注
拨打电话
留言咨询