Sequencing Tryptic Peptides Using Sulphonated Derivatives

2003-07-04 16:07  下载量:121

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PSD can be used in MALDI−TOFMS to fragment and so sequence a peptide. Fragmentation requires the free movement of a proton along the peptide backbone. Tryptic peptides are difficult to fragment due to their proton sequestering C−terminal (lysine or arginine). A new and novel derivitization places a sulphonated group at the peptides N−terminal. This negatively charged group counteracts the positive group of Lys/Arg. As a result fragmentation via proton mobilisation is improved. We describe here examples of this so called chemically activated fragmentation (CAF).

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