方案摘要
方案下载| 应用领域 | 生物产业 |
| 检测样本 | 其他 |
| 检测项目 | |
| 参考标准 | none |
Biofilms are defined as “bacteria growing on a surface as single or multi-layered communities”. Over the past several years the importance of biofilms in research has been increasingly growing as scientists realized that many, if not a large majority, of microorganisms exist naturally as biofilms. Indeed, biofilms studies are common in water science (natural waters or waste waters), environmental studies (biofilm formation on rocks), material science (antifouling surfaces) and finally medical or biomedical studies (infections, implantology). The study of biofilms has evolved with the development of many staining, molecular, or microscopy techniques. However, most of these techniques are destructive and therefore only provide endpoint measurements. Consequently, the metabolism and the dynamic behavior of biofilms remains mostly inaccessible to researchers. In this context, isothermal microcalorimetry is a valuable tool enabling investigation of development and metabolic activities of growing or mature biofilms. Several techniques combined with an isothermal calorimeter can be used to study biofilms and this application note aims at summarizing these techniques and providing some tips and tricks.
Biofilms are defined as “bacteria growing on a surface as single or multi-layered communities”. Over the past several years the importance of biofilms in research has been increasingly growing as scientists realized that many, if not a large majority, of microorganisms exist naturally as biofilms. Indeed, biofilms studies are common in water science (natural waters or waste waters), environmental studies (biofilm formation on rocks), material science (antifouling surfaces) and finally medical or biomedical studies (infections, implantology).
The study of biofilms has evolved with the development of many staining, molecular, or microscopy techniques. However, most of these techniques are destructive and therefore only provide endpoint measurements. Consequently, the metabolism and the dynamic behavior of biofilms remains mostly inaccessible to researchers.
In this context, isothermal microcalorimetry is a valuable tool enabling investigation of development and metabolic activities of growing or mature biofilms. Several techniques combined with an isothermal calorimeter can be used to study biofilms and this application note aims at summarizing these techniques and providing some tips and tricks.
利用微量热技术研究病毒结构与宿主细胞受体结合反应
等温量热法研究水泥水化
等温微量热法研究生物制品长时间稳定性评价
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