方案摘要
方案下载| 应用领域 | |
| 检测样本 | |
| 检测项目 | |
| 参考标准 |
The medicinal plant Atractylodes macrocephala (Baizhu in Chinese) has been widely used in traditional Chinese medicine for energy and stomach complaints, treatment of dyspepsia and anorexia, anti-inflammation, anticancer and for increasing assimilation. A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation and purification of two main bioactive components,namely, atractylon and atractylenolide III from A. macrocephala by using light petroleum (60–908C)–ethyl acetate–ethanol–water (4 :1:4:1 v/v) as the two-phase solvent system in dual-mode elution. Compared with the separation using the normal-mode elution, the dual-mode HSCCC can be achieved with shorter elution time.Atractylenolide III (32.1 mg) at 99.0% purity and 319.6 mg atractylon at 97.8% purity could be obtained from 1000 mg crude sample in a single run. The recoveries of atractylenolide III and atractylon were 95.4 and 92.6%, respectively.
文献贡献者
上海同田生物技术：运用高速逆流色谱法从仙茅中分离仙茅甙和仙茅甙乙
上海同田生物技术：运用高速逆流色谱和ELSD联用技术从Adenophora tetraphlla（南沙参）根茎中分离化学成分
上海同田生物技术：高速逆流色谱法分离制备丹酚酸B
相关产品
关注
拨打电话
留言咨询
