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Abstract： Pseudostellarin B (cyclic peptide) was isolated and purified from the herbs of Pseudostellaria heterophylla (Miq.)Pax for the first time by high-speed counter-current chromatography(HSCCC)using a two-phase solvent system consisting of n-butanol-ethyl acetate-water(0.6:4.4:5,v/v).The technique can isolate mg levels of the target compound per run with purity better than 96%. The chemical structure of the compound has been positively confirmed by electrospray ionization time of flight(TOF) MS,H-NMR,C-NMR and H-C COSY analyses.
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