全自动液液萃取——VERSA1100

加拿大欧罗拉生物科技有限公司

2019/12/11 10:36

液-液萃取（LLE）法是油脂组学研究中常用的质谱样品制备方法。VERSA 1100自动液–液萃取工作站已配置为使用氯仿：甲醇（Bligh and Dyer或Folch）方法从生物样品中提取脂质，以减少人为误差并提高效率。

Liquid-liquid extraction (LLE) method is commonly used for mass spectrometry sample preparation in lipidomics research. The VERSA 1100 Automated Liquid-Liquid Extraction Workstation has been configured to extract lipids from biological samples using the chloroform:methanol (Bligh and Dyer or Folch) method to reduce human error and increase efficiency.

VERSA-1100-Automated-Lipid-Extraction-Efficiency-400x250.jpg

以下是从鸡肝样本中提取脂质的数据,样品在15x85mm玻璃管中以24个样品/批次提取。该工艺包括添加溶剂、混合、相分离、干燥、重组、添加内标和样品转移。与手工氯仿：甲醇脂肪提取实验相比，用VERSA 1100提取鸡肝前200位脂肪的自动提取效率为94.3%，变异系数（CV）为手工提取的2倍。VERSA 1100自动油脂提取工作站也与非氯化溶剂系统（如MTBE和BUME方法）兼容

Below are the data generated from extracting lipids from chicken liver samples. In brief, samples are extracted in 15x85mm glass tubes in 24 sample/batch. This automated procedure includes adding solvents, mixing, phase separation, drying, reconstitution, adding internal standard and sample transfer. Compared to manual chloroform:methanol lipid extraction experiment, the automated extraction efficiency was found to be 94.3% and the coefficient of variation (CV) for the extraction of top 200 lipids from chicken liver using VERSA 1100 was 2-fold lower than that obtained with manual extraction. The VERSA 1100 Automated Lipid Extraction workstation is also compatible with non-chlorinated solvent systems such as MTBE and BUME methods.

文章来源：加拿大欧罗拉生物科技官网

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