资料摘要
资料下载On-line measurement of dissolved O2 in shake-flasks was realized via immobilized sensor spots containing a fluorophore with an O2 dependent luminescent decay time. An unaffected sensor signal during 80 autoclaving cycles suggests multi-usage of sensor equipped shake-flasks. The sensor had a response time of 6 s. Quantification of gas-liquid mass transfer revealed maximum kLa values of 150 h−1, from which maximum O2 transfer capacity of 33 mM h−1 was calculated. Liquid volume and shaking frequency have a strong influence on kLa. Exemplified by cultivations of Corynebacterium glutamicum the importance of shaking rate for O2 supply of bacterial cultures is shown. Sampling of microbial cultures with intermittent shaking of a few minutes can cause O2 limitation. Based on the results of this work a simple and straightforward tool is now available for accurate O2 sensing in shake-flasks, which are widely used in microbial cultivations.
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光电测量资料
简介：博源光电目前有以下几款光电测量装置：拉曼测量装置、激光测量装置、LED测量装置、膜厚测量装置、辐照度测量套件，感兴趣的请下载宣传页进行查看。
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简介：博源光电目前有以下几个系列的功率计：光电功率探测器、热电堆功率探测器、积分球功率探测器，感兴趣的请下载宣传页进行查看。
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简介：博源光电目前有以下几个系列的光源：氘卤光源、均匀光源、LED光源、弧灯光源，感兴趣的请下载宣传页进行查看。
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简介：博源光电目前有以下几个系列的光谱仪：微型光谱仪、高分辨率光谱仪、高灵敏光谱仪、近红外光谱仪、拉曼光谱仪，感兴趣的请下载宣传页进行查看。
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