牛里脊肉中蛋白质和脂肪含量的检测

2023/03/19   下载量: 0

方案摘要

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应用领域 食品/农产品
检测样本 畜禽肉及副产品
检测项目 营养成分>脂肪, 蛋白质
参考标准 GB/T 5009.6 食品中脂肪的测定

不同管理的巴厘岛屠宰场(RPH)的巴厘牛肉屠宰质量Quality of Bali Beef Cut on Different Management of Slaughterhouses (RPH) in Bali Province

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不同管理的巴厘岛屠宰场(RPH)的巴厘牛肉屠宰质量Quality of Bali Beef Cut on Different Management of Slaughterhouses (RPH) in Bali Province


2 grams of meat sample were weighed and wrapped in filter  paper and put in a lead, dried in an oven for 9 hours at a temperature of 1050C. The soxtherm tube was dried in an  oven for 3 hours at 1050C, then cooled in a desiccator and weighed (B). The lead containing the sample after being  dried was put into a soxtherm tube, filled the soxtherm tube with 200 ml of n-Hexane until the sample was  completely immersed. Extraction for 4 hours in a soxtherm apparatus, then air dry the soxtherm tube in a forced oven  for 15 minutes then dry for 3 hours in a dry oven at 1050C, cool in a desiccator for 30 minutes, weigh the soxtherm  tube containing fat extract.Photo_HYDROTHERM + SOXTHERM, 1.jpg

0.3克样品,1克催化剂,5毫升浓硫酸,消化1.5小时。

A total of 0.3 grams of sample, was placed in a vapodest tube and added 1 grain of selenium catalyst and 5 ml of  concentrated H2SO4, then carried out destruction (heating in a boiling state) for 1.5 hours until the solution is clear.  After cooling, 50 ml of distilled water and 20 ml of 40% NaOH were added, then distilled. The results of the  distillation were accommodated in an Erlenmeyer flask containing a mixture of 20 ml H3BO3 and 2 drops of pink  green bromine cresol. After the distillate volume (distillate) became 100 ml and turned bluish in color, the distillation  was stopped and the distillate was titrated with 0.1 N HCL until pink.



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