方案摘要
方案下载应用领域 | 医疗/卫生 |
检测样本 | 内脏器官 |
检测项目 | 生化检验>蛋白 |
参考标准 | / |
高钠饮食可将大鼠肾蛋白多糖转化为促炎介质High sodium diet converts renal proteoglycans into pro-inflammatory mediators in rats
高钠饮食可将大鼠肾蛋白多糖转化为促炎介质High sodium diet converts renal proteoglycans into pro-inflammatory mediators in rats
Measurements of renal and dermal sodium
Cortical renal tissue was obtained by cutting a triangular shaped sample from the cortical area of the kidney. Abdominal skin tissue was obtained and shaved before sodium measurements. Samples were cut in half and the wet weight of each part was measured. Per sample, both halves were dried overnight at 80˚C, dry weight was measured and one of the halves solved in pure nitric acid (Sigma-Aldrich, St Louis, USA) for sodium measurements. The second half of the sample was used to calculate the amount of protein per sample by measuring the nitrogen content according to Dumas using the Gerhardt Dumatherm Nitrogen/Protein analyser (C. Gerhardt UK Ltd, Northamptonshire, UK). Sodium concentrations were measured by atomic absorption (flame) spectrometry (Thermo M Series AA Spectrometer, Thermo Fisher Scientific, Waltham, USA) and expressed per dry weight and per nitrogen content.
工业园区污水及经生物反应器处理后的污水氨氮、有机氮及化学需氧量(COD)的测定
使用格哈特凯氏定氮仪测定药用汉麻(大麻)中氮含量
使用格哈特凯氏定氮仪测定水溶性复合氮磷肥料NPF中氮含量
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