资料摘要
资料下载The atomic force microscope is a high-resolution scanning-probe instrument which has become an important tool for cellular and molecular biophysics in recent years but lacks the time resolution and functional specificities offered by fluorescence microscopic techniques. To exploit the advantages of both methods, here we developed a spatially and temporally synchronized total internal reflection fluorescence and atomic force microscope system. The instrument, which we hereby call STIRF-AFM, is a stage-scanning device in which the mechanical and optical axes are coaligned to achieve spatial synchrony. At each point of the scan the sample topography (atomic force microscope) and fluorescence (photon count or intensity) information are simultaneously recorded. The tool was tested and validated on various cellular (monolayer cells in which actin filaments and intermediate filaments were fluorescently labeled) and biomolecular (actin filaments and titin molecules) systems.Wedemonstrate that with the technique, correlated sample topography and fluorescence images can be recorded, soft biomolecular systems can be mechanically manipulated in a targeted fashion, and the fluorescence of mechanically stretched titin can be followed with high temporal resolution.
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水凝胶机械性能测试
简介：The Piuma Nanoindenter is a new and versatile instrument for the characterization of the mechanical properties of soft hydrogels. Check our recently released application note on agarose and gelatin to get an impression about some essential measurements the Piuma can perform! And it does not stop here: very soft hydrogels, other biomaterials, tissues, cell sheets and even single cells are all part of the Piuma domain, which provides researchers with unparalleled accuracy and ease of use in mechanical experiments.利用piuma生物纳米压痕研究水凝胶的力学性质，包括杨氏模量，表面形貌等性质。
骨水泥的杨氏模量测试
简介：Biosilica, a biocompatible, natural inorganic polymer that is formed by an enzymatic, silicatein-mediated reaction in siliceous sponges to build up their inorganic skeleton, has been shown to be morphogenetically active and to induce mineralization of human osteoblast-like cells (SaOS-2) in vitro. In the present study, we prepared beads (microspheres) by encapsulation of β-tricalcium phosphate [β-TCP], either alone (control) or supplemented with silica or silicatein, into the biodegradable copolymer poly(D,L-lactide-co-glycolide) [PLGA]. Under the conditions used, ≈5% β-TCP, ≈9% silica, and 0.32 μg/mg of silicatein were entrapped into the PLGA microspheres (diameter≈800 μm). Determination of the biocompatibility of the β-TCP microspheres, supplemented with silica or silicatein, revealed no toxicity in the MTT based cell viability assay using SaOS-2 cells. The adherence of SaOS-2 cells to the surface of silica-containing microspheres was higher than for microspheres, containing only β-TCP. The formation of new bone induced by the microspheres is also evident from measurements of the stiffness/reduced Young's modulus of the regenerated bone tissue. The reduced Young's modulus of the regenerating bone tissue around the implants was markedly higher for the silica-containing microspheres (1.1 MPa), and even more for the 1:1 mixture of the silica- and silicatein-containing microspheres (1.4 MPa), compared to the β-TCP microsphere controls (0.4 MPa). We propose that based on their morphogenetic activity on bone-forming cells in vitro and the results of the animal experiments presented here, silica/ biosilica-based scaffolds are promising materials for bone repair/regeneration.
纳米压痕仪与压电力显微镜联用
简介：纳米压痕仪与压电力显微镜联用 结合纳米压痕的压电力显微镜测试（详见PFM应用手册）  力反馈 (应力控制)以及压痕深度(应力控制)  力学实时分析  力学 vs 位置  恒幅载荷  准静态实验法: 压痕蠕变, 应力松弛  动态测试
纳米压痕仪资料PPT
简介：Asylum’s MFP Nanoindenter 纳米压痕仪是一款为市场所认可的商业化纳米级压痕仪，是业内第一款基于原子力显微镜的压痕仪器。通过结合顶级原子力显微镜的传感器，为该款纳米压痕模块提供了超过其他纳米压痕系统所无法企及的准度、精度以及敏感度。 纳米压痕仪通过与原子力显微镜的结合，利用原子力显微镜卓越的形貌观察能力同时对压头和压痕结果的测试，可以提供独一无二的定量压痕接触区域计算能力，提供业内最准确的确定接触区域 ，获得间接估算材料性质所没有的精度。特有的一维柔性铰链的被动驱动方式从而使得漂移和深度测试中的其他错误得到最小化。压入过程中的热漂移效应全自动扣除，测量结果更加真实、可靠。 高性能的样品加载系统和传感技术使得nanoindeter纳米压痕仪成为了最多样化的纳米力学表征工具，是科学家、工程师和其他各领域用户的最佳选择。在摩擦、模量成像、动态力学分析、薄膜、陶瓷、复合物、聚合物、微机电系统、生物和金属等领域都有广泛应用。符合 ISO/IEC 17025.1以及ISO 14577-22国际标准。 行业应用：材料、生物、化学、物理相关的政府检测机构、科研院所、高校和大型企业研究机构
纳米压痕仪操作手册
简介：Asylum’s MFP Nanoindenter 纳米压痕仪是一款为市场所认可的商业化纳米级压痕仪，是业内第一款基于原子力显微镜的压痕仪器。通过结合顶级原子力显微镜的传感器，为该款纳米压痕模块提供了超过其他纳米压痕系统所无法企及的准度、精度以及敏感度。 纳米压痕仪通过与原子力显微镜的结合，利用原子力显微镜卓越的形貌观察能力同时对压头和压痕结果的测试，可以提供独一无二的定量压痕接触区域计算能力，提供业内最准确的确定接触区域 ，获得间接估算材料性质所没有的精度。特有的一维柔性铰链的被动驱动方式从而使得漂移和深度测试中的其他错误得到最小化。 高性能的样品加载系统和传感技术使得nanoindeter纳米压痕仪成为了最多样化的纳米力学表征工具，是科学家、工程师和其他各领域用户的最佳选择。在摩擦、模量成像、动态力学分析、薄膜、陶瓷、复合物、聚合物、微机电系统、生物和金属等领域都有广泛应用。 行业应用：材料、生物、化学、物理相关的政府检测机构、科研院所、高校和大型企业研究机构 纳米压痕仪操作手册
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