Surface enhanced Raman scattering analyses of individual silver nanoaggregates on living single yeast cell wall

针尖加强拉曼光谱活细胞成像——We labeled the living yeast cell surface
Saccharomyces cerevisiae strain W303-1A by silver nanoparticles which can form nanoaggregates and found to show surface enhanced Raman scattering
SERS activity. Blinking of SERS and its polarization dependence reveal that SERS signals are from amplified electromagnetic field at nanometric Ag nanoparticles gaps with single or a few molecules sensitivity. We tentatively assigned SERS spectra from a yeast cell wall to mannoproteins. Nanoaggregate-by-nanoaggregate variations and temporal fluctuations of SERS spectra are discussed in terms of inhomogeneous mannoprotein distribution on a cell wall and possible ways of Ag nanoaggregate adsorption, respectively