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浙江大学的叶老师领导下的研究团队,应用美国FIAlab公司生产的FIAlab-3500顺序注射分析仪研究开发出一款《平面多电极阵列神经元芯片嗅觉感受器》,有关该产品的介绍文章已发表在2010年9月的“生物传感器与生物电子学”杂志上。为了大家学习参考,现将她刊登在我公司的网站上。 Abstract A novel olfactory neurochip based on olfactory receptor neurons (ORNs) cultivated on the surface of the 60-channel planar multi-electrode array (MEA) devices was developed in this study. In order to investigate the odor-response characteristics of ORNs, two types of odorants at different concentrations were quantitatively pumped into the neurochip by a customized gas intake system, and the extracellular electrical activities of multiple ORNs were simultaneously recorded in vitro. Accordingly, the odor-response features of ORNs such as firing amplitude, firing threshold, firing rate as well as firing channels were analyzed qualitatively and quantitatively in terms of ORN spike trains. Especially, after introducing the classification algorithm based on the spike threshold, the odor-response maps from the multiple sites could be used to identify dl-limonene (LIM) and isoamyle acetate (ISO) odorants. These preliminary studies indicate that the ORN-based biosensor developed here has a potential capability of distinguishing different odorants as a true bionic electronic nose.
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