资料摘要
资料下载可用于麻醉学研究的动物种类很多,有关实验动物学的专著中都有较详细的叙述。本节仅就麻醉学研究中最常用的动物做简单的介绍。 一、大鼠 大鼠遇惊扰时常试图撕咬,处理不当会咬人致伤。同类间好斗,最好不同笼饲养。大鼠寿命在2-3年之间,约90天进入成年期。成年大鼠体重在200-400g,总血量为体重的6-8%。大鼠有尾静脉3条,背侧1条、腹侧2条,均可供静脉穿刺。大鼠实验常用有机玻璃筒完成,简便易行。一般体重在200-300g的大鼠,圆筒的内径为6cm左右,使之在内无法转身。头尾端均盖口径合适的橡胶塞,既可用于通气,也可用于穿过鼠尾。大鼠麻醉的判断标准有:前爪直立位消失、前爪翻正反射消失、夹尾无体动反应。 二、小鼠 小鼠性情温和。寿命2-3年,约80天进入成年期。成年小鼠体重30-50g。总血量为体重的6-7%。小鼠实验给药多为腹腔注射,但也可尾静脉穿刺注射给药。小鼠尾静脉解剖与大鼠相同。 三、家兔 性情温顺,便于饲养。依种类不同,成年兔体重在1.5-8kg。寿命7-10年,8个月进入成年期。总血量约为体重的5-8%。家兔耳缘静脉清晰、固定,易行静脉穿刺。按压静脉近心端,使静脉膨胀,常可穿刺成功。家兔声门较高,对成年家兔用小号直喉镜多可在明视下插入3.5F气管插管。现有国产小动物呼吸机,可经气管插管以30-50ml潮气量、25-30次/分钟呼吸频率行机械通气,同时实施吸入麻醉。 四、狗 狗的品种很多,体型悬殊。寿命约为15年。2岁进入成年期。总血量占体重的7%。在药代动力学研究中,狗的麻醉常选用硫喷妥钠,一次静脉注射 25mg/kg维持麻醉45分钟。吸入麻醉剂维持适当的麻醉深度。狗口腔明视气管插管较容易,麻醉后置于仰卧位,头后仰,颈部伸直。可使口腔与喉头气管成直线。将狗舌拉出,置入喉镜可见会厌。向上挑起,暴露声门,插入气管导管。
斑马鱼胚胎显微注射实验操作方法
简介:斑马鱼显微注射是将实验材料直接注射到1-4细胞期的斑马鱼胚胎中,由于在这个胚胎发育早期没有膜分隔细胞和卵黄,注入1个细胞或卵黄的溶液将扩散到整个胚胎中。通过注射不同类型的实验样品,实现基因的瞬时过表达(over-expression)、表达敲减(knock-down)、以及制备转基因或突变斑马鱼品系等研究。
桌式麻醉机使用说明
简介:吸入式气体麻醉,是在供给动物的气流中添加入麻醉气体(浓度受麻醉挥发罐的精确控制,且可随动物生理状况实时调节),以此对动物进行麻醉。 在维持麻醉过程前,需要以高麻醉浓度、大流量的气流对动物进行诱导麻醉,方便对动物进行固定处理等术前准备。 麻醉尾气需通入活性炭盒(F/AIR)进行吸收处理,以消除其对实验人员的影响。
脑立体定位仪的使用方法
简介:脑立体定位仪是利用颅骨外面的标志或其他参考点所规定的三维坐标系统来确定皮层下某些神经结构的位置,以便在非直视暴露下对其进行定向的刺激、破坏、注射药物或引导电位等操作。 它是神经解剖、神经生理、神经药理和神经外科等领域内重要的研究设备,可用于帕金森氏病动物模型的建立、电线动物模型的建立、脑内肿瘤动物模型的建立、学习记忆、神经干细胞移植、脑缺血等研究。
Mixture/Dose Delivery System
简介:The PHD ULTRA? Gradient Systems allows you to quickly and easily run binary or ternary mixtures automatically without a PC. The Method is totally programmed from a master controller pump, for easy set-up. The intuitive program interface makes simple to complex mixing easy. The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream. The flow can be smooth and continuous or stepped. No stopping the experiment, mixing different % volume mixtures and placing new syringes into the pumps. Push one button and it all happens automatically. Binary and ternary gradient systems are available with or without a stand. Individual Satellite units are also available for purchase separately. For more information on the PHD ULTRA? Syringe Pumps see page 18-22. For more information on the PHD ULTRA? Satellite units see page 21.
harvard pump 11 pico plus使用手...
简介:General Information Serial Number All inquires concerning our product should refer to the serial number of the unit. Serial numbers are located on the rear of the chassis. Calibration All syringe pumps are designed and manufactured to meet their performance specifications at all rated voltages and frequencies. A calibration certificate is available upon request. Contact customer service for details and pricing. Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to the original customer purchaser. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation may not apply to you.
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