资料摘要
资料下载不需显微操作体细胞核移植的利益和前景远大。当前的成果包括降低装备费用，简化预备工作及实验技术要求低。任何做过胚胎方面试验和口吸管方面工作的人都能很快掌握。尽管用卵母细胞试验的直接工作没有显著减少，但降低了这些工作的技术难度。此外，与早期的技术相比，预备工作如制作工具可以省略。然而，应该注意到产生三倍体需要较多的卵母细胞。无透明带的胚胎可能有利于嵌合体的制作，克隆方法的简化也将有利于核移植的自动化。在卵母细胞成熟期间，用于建立该方法的供 体细胞是贴壁的原代培养的颗粒细胞，这些细胞并不是都很适合做供体。随着方法的进一步改进，可能能用不同器官组织的传代细胞和血清饥饿培养的细胞。
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斑马鱼胚胎显微注射实验操作方法
简介：斑马鱼显微注射是将实验材料直接注射到1-4细胞期的斑马鱼胚胎中，由于在这个胚胎发育早期没有膜分隔细胞和卵黄，注入1个细胞或卵黄的溶液将扩散到整个胚胎中。通过注射不同类型的实验样品，实现基因的瞬时过表达（over-expression）、表达敲减（knock-down）、以及制备转基因或突变斑马鱼品系等研究。
桌式麻醉机使用说明
简介：吸入式气体麻醉，是在供给动物的气流中添加入麻醉气体（浓度受麻醉挥发罐的精确控制，且可随动物生理状况实时调节），以此对动物进行麻醉。 在维持麻醉过程前，需要以高麻醉浓度、大流量的气流对动物进行诱导麻醉，方便对动物进行固定处理等术前准备。 麻醉尾气需通入活性炭盒（F/AIR）进行吸收处理，以消除其对实验人员的影响。
脑立体定位仪的使用方法
简介：脑立体定位仪是利用颅骨外面的标志或其他参考点所规定的三维坐标系统来确定皮层下某些神经结构的位置，以便在非直视暴露下对其进行定向的刺激、破坏、注射药物或引导电位等操作。 它是神经解剖、神经生理、神经药理和神经外科等领域内重要的研究设备，可用于帕金森氏病动物模型的建立、电线动物模型的建立、脑内肿瘤动物模型的建立、学习记忆、神经干细胞移植、脑缺血等研究。
Mixture/Dose Delivery System
简介：The PHD ULTRA? Gradient Systems allows you to quickly and easily run binary or ternary mixtures automatically without a PC. The Method is totally programmed from a master controller pump, for easy set-up. The intuitive program interface makes simple to complex mixing easy. The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream. The flow can be smooth and continuous or stepped. No stopping the experiment, mixing different % volume mixtures and placing new syringes into the pumps. Push one button and it all happens automatically. Binary and ternary gradient systems are available with or without a stand. Individual Satellite units are also available for purchase separately. For more information on the PHD ULTRA? Syringe Pumps see page 18-22. For more information on the PHD ULTRA? Satellite units see page 21.
harvard pump 11 pico plus使用手...
简介：General Information Serial Number All inquires concerning our product should refer to the serial number of the unit. Serial numbers are located on the rear of the chassis. Calibration All syringe pumps are designed and manufactured to meet their performance specifications at all rated voltages and frequencies. A calibration certificate is available upon request. Contact customer service for details and pricing. Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to the original customer purchaser. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation may not apply to you.
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