资料摘要
资料下载显微操作技术包括细胞核移植、显微注射、嵌合体技术、胚胎移植以及显微切割等,例如多莉羊就是运用细胞核移植技术而成功的;而转基因技术指的是将外源基因导入体细胞并能稳定的嵌入宿主动物的生殖细胞染色体中的一门技术,基因转殖动物被定义为由人为的方式将外源基因引入体内而引起基因改变的动物,并可将遗传特质传递到接续的每一世代中。 微注射应用的范围非常广泛,从辅助(体外)细胞受精技术至分子和细胞基本组分的转运都需使用这一技术,比较典型的是将某些物质注射进细胞中以操作和/或监测某种特定的存活细胞中的基本机体生物化学状态。这些可以注射进细胞的物质包括有:各种细胞器、激酶、组织化学标志物(比如辣根过氧化物酶或者荧光黄)、蛋白质、代谢物质、微磁头、离子、抗体、基因、分子生物学的mRNA和DNA等等。运用这一技术,也可以实现用于单个细胞或一组细胞的较少量(皮升至毫升)药剂或药物的精确输送(微灌注),例如药理学的药物检验。转基因动物的制作,可以利用基因微注射(gene microinjection)、胚干细胞(embryonic stem cells,ES cells)、精子载体(sperm vector)、反转录病毒感染(retroviral vectorinfection)及体细胞核移置(somatic cell nucleartransfer)等方法达成,其中显微注射为目前应用最普遍之方法之一。
斑马鱼胚胎显微注射实验操作方法
简介:斑马鱼显微注射是将实验材料直接注射到1-4细胞期的斑马鱼胚胎中,由于在这个胚胎发育早期没有膜分隔细胞和卵黄,注入1个细胞或卵黄的溶液将扩散到整个胚胎中。通过注射不同类型的实验样品,实现基因的瞬时过表达(over-expression)、表达敲减(knock-down)、以及制备转基因或突变斑马鱼品系等研究。
桌式麻醉机使用说明
简介:吸入式气体麻醉,是在供给动物的气流中添加入麻醉气体(浓度受麻醉挥发罐的精确控制,且可随动物生理状况实时调节),以此对动物进行麻醉。 在维持麻醉过程前,需要以高麻醉浓度、大流量的气流对动物进行诱导麻醉,方便对动物进行固定处理等术前准备。 麻醉尾气需通入活性炭盒(F/AIR)进行吸收处理,以消除其对实验人员的影响。
脑立体定位仪的使用方法
简介:脑立体定位仪是利用颅骨外面的标志或其他参考点所规定的三维坐标系统来确定皮层下某些神经结构的位置,以便在非直视暴露下对其进行定向的刺激、破坏、注射药物或引导电位等操作。 它是神经解剖、神经生理、神经药理和神经外科等领域内重要的研究设备,可用于帕金森氏病动物模型的建立、电线动物模型的建立、脑内肿瘤动物模型的建立、学习记忆、神经干细胞移植、脑缺血等研究。
Mixture/Dose Delivery System
简介:The PHD ULTRA? Gradient Systems allows you to quickly and easily run binary or ternary mixtures automatically without a PC. The Method is totally programmed from a master controller pump, for easy set-up. The intuitive program interface makes simple to complex mixing easy. The Gradient Profile is used to combine multiple flow streams from different pumps into a common output stream. The flow can be smooth and continuous or stepped. No stopping the experiment, mixing different % volume mixtures and placing new syringes into the pumps. Push one button and it all happens automatically. Binary and ternary gradient systems are available with or without a stand. Individual Satellite units are also available for purchase separately. For more information on the PHD ULTRA? Syringe Pumps see page 18-22. For more information on the PHD ULTRA? Satellite units see page 21.
harvard pump 11 pico plus使用手...
简介:General Information Serial Number All inquires concerning our product should refer to the serial number of the unit. Serial numbers are located on the rear of the chassis. Calibration All syringe pumps are designed and manufactured to meet their performance specifications at all rated voltages and frequencies. A calibration certificate is available upon request. Contact customer service for details and pricing. Warranty Harvard Apparatus warranties this instrument for a period of two year from date of purchase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material. This warranty does not extend to damage resulting from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends only to the original customer purchaser. IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES. Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, OR FITNESS FOR A PARTICULAR USE, OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation may not apply to you.
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